Cell Transient Transfection Workstation
This application note describes an automated small scale transient transfection system. Cells have to be transfected with different type of DNA constructs in order to produce specific proteins. The cell culture is divided from a 3L spinner flask to 48 CultiFlasks. These CultiFlasks were stored for several days in an incubator.
In this project, our customer has presented us with specific requirements. They need a device that can maintain a sterile environment for handling open containers/tubes, which calls for safety class 1 product protection. Furthermore, they require the capability to achieve a complete CultiFlask setup, involving pipetted DNA, Transfection reagent, and cells together, all within one hour on the system.
The focus of this application note is an automated small-scale transient transfection system. The objective is to transfect cells with various DNA constructs to produce specific proteins. The process involves dividing the cell culture from a 3L spinner flask into 48 CultiFlasks, which are subsequently stored in an incubator for several days.
Cell Transient Tranfection by Synchron's Xperimate
Day 1. Transient Transfection
Two different DNA constructs and transfection reagent were pipetted in media into a CultiFlask. After short incubation cell suspension is added and the CultiFlask will be stored in an incubator overnight.
Day 2. Adding Feeding Solutions
Three different feeding solutions were added to the cell suspension into the CultiFlask. The CultiFlask will be stored in an incubator for 4 days.
Day 5. Measuring Yield
From each CultiFlask a sample is taken and added to an 96w MTP. If the yield is enough the CultiFlasks will be harvested by centrifugation (300g) and a filtration step 0.22µm, all steps automatically performed by Synchrons’ Robotic platform.
IF WE CAN THINK IT, WE CAN BUILD IT
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Interested in exploring the potential of automating your workflow with Synchrons' customized solutions like the transient transfection system? Feel free to reach out to us for more details!
Key Features
Synchron Xperimate x-y-z robotic platform
2.50 meter system with 2.0 meter deck and 0.50 meter overhang for getting access to robotic centrifuge
Robotic arm with 4 channels, 2 channels equipped with SX48 micro-annular gear pumps, 2 others equipped with SX3 micro- annular gear pumps
2 channels equipped with custom made pipetting tips so that they can pipette directly onto 0.22µm Luor-Lock filters, 2 others equipped with disposable tips
Flexispan
Chemical resistant tubing, valves and pumps
2nd robotic arm with tube gripping tool
Fully integrated 2D Tube scanner by Ziath
Fully integrated Shaker equipped with 48 position tube rack
Fully integrated De-/Capping module
Fully integrated dispensing module for adding cell suspension
Fully integrated robotic centrifuge by Hettich
Magnetic stirrer for Spinnerflask by IKA
Peristaltic pump
Customized rack for 24 positions 0.22µm Luor-Lock filters
Customized racks for 24 positions 50mL Tubes
